mdr k pneumoniae strain  (ATCC)

Journal: ACS infectious diseases

Article Title: Restoring and enhancing the potency of existing antibiotics against drug-resistant Gram-negative bacteria through the development of potent small-molecule adjuvants

doi: 10.1021/acsinfecdis.2c00121

Figure Lengend Snippet: (A and B) Checkerboard assays showed sensitization of A. baumannii by MD-124 towards rifampicin and novobiocin. (C and D) Checkerboard assays showed sensitization of K. pneumoniae by MD-124 towards rifampicin and clarithromycin. (E) Sensitization of an NDM-1-expressing strain of E. coli by MD-124 towards rifampicin. (F) Sensitization of an mcr-1-expressing strain of E. coli by MD-124 towards rifampicin. (G) Sensitization of MDR K. pneumoniae by MD-124 towards rifampicin. (H) Sensitization of MDR S. Typhimurium by MD-124 towards rifampicin. All experiments were done at least in biologically independent duplicate.

Article Snippet: Thus, MD-124 was tested on one MDR K. pneumoniae strain (ATCC BAA-2472) and one MDR S. Typhimurium strain (ATCC 14028), both of which are resistant to almost all antibiotics.

Techniques: Expressing

Journal: International Journal of Molecular Sciences

Article Title: Current State of Knowledge Regarding WHO High Priority Pathogens—Resistance Mechanisms and Proposed Solutions through Candidates Such as Essential Oils: A Systematic Review

doi: 10.3390/ijms24119727

Figure Lengend Snippet: Studies assessing the antimicrobial activity of essential oils against methicillin resistant, vancomycin-intermediate and -resistant S. aureus (a non-exhaustive list).

Article Snippet: Gadisa E et al., 2019 , MRSA E. coli —MDR strain K. pneumoniae —MDR strain S. aureus ATCC 25922 E. coli ATCC 25922 K. pneumoniae ATCC 700603 , Blepharis cuspidata Qoree waraantii Boswellia ogadensis Vollesen Thymus schimper used in combination , Broth microdilution assay FIC index , The combination of B. cuspidata and T. schimperi EOs highlighted a MIC/MBC = 0.39 μg/mL against MRSA. Moreover, association of B. cuspidata and B. ogadensis had a MIC of 1.56 μg/mL for MRSA. A FICI of 0.38 (a synergistic effect) was obtained for MRSA using B. cuspidata + B. orgadensis , and of 0.25 for K. pneumoniae MDR, for the same association. , [ ] .

Techniques: Activity Assay, Diffusion-based Assay, Inhibition, Cytotoxicity Assay, Electron Microscopy, In Vitro, Dilution Assay, Preserving, Quantitative Proteomics, Nucleic Acid Electrophoresis, Membrane, Confocal Laser Scanning Microscopy, Concentration Assay, Titration, Bacteria, Microscopy, Transmission Assay, Microdilution Assay, Produced, Modification, Clinical Proteomics, Blocking Assay, Staining, Cell Culture, Fourier Transform Infrared Spectroscopy, Spectroscopy, Reverse Transcription, Real-time Polymerase Chain Reaction, Crystal Violet Assay, Expressing, Flow Cytometry, In Vivo, Liposomes, Time-Kill Assay, Formulation, MTT Assay, Incubation, Thin Layer Chromatography, Bioassay, Antibiofilm Assay, Resazurin Assay, Biofilm Production Assay, Control, Infection, Cream, Antioxidant Activity Assay, Permeability, Virus, Extraction, Isolation

Journal: Frontiers in Pharmacology

Article Title: In silico genome-scale metabolic modeling and in vitro static time-kill studies of exogenous metabolites alone and with polymyxin B against Klebsiella pneumoniae

doi: 10.3389/fphar.2022.880352

Figure Lengend Snippet: MICs of K. pneumoniae isolates.

Article Snippet: With the aim of identifying promising metabolite adjuvants to increase antimicrobial activity of polymyxin B against K. pneumoniae , we have studied polymyxin-resistant strain ATCC 10031, polymyxin-susceptible strain ATCC 700721, and polymyxin-susceptible but MDR K. pneumoniae strains (ATCC 700603 and BAA-2146) ( ) .

Techniques:

Journal: Antibiotics

Article Title: Heteroaryl-Ethylenes as New Lead Compounds in the Fight against High Priority Bacterial Strains

doi: 10.3390/antibiotics10091034

Figure Lengend Snippet: MIC and MBC values for Gram-negative control strains.

Article Snippet: Control strains S. aureus ATCC29213, S. aureus ATCC12598, S. aureus ATCC BAA-1556 (USA300), S. aureus ATCC 700699 (Mu50-NRS1), E. faecalis ATCC29212, E. faecalis ATCC51299, A. baumannii ATCC17978; E. coli ATCC25922, P. aeruginosa ATCC27853, K. pneumoniae ATCC700603; and the MDR strain K. pneumoniae ATCC BAA-2814 were obtained from the American Type Culture Collection (Manassas, VA, USA).

Techniques: Control

Journal: Journal of Antimicrobial Chemotherapy

Article Title: Restoring carbapenem efficacy: a novel carbapenem companion targeting metallo-β-lactamases in carbapenem-resistant Enterobacterales

doi: 10.1093/jac/dkaa455

Figure Lengend Snippet: Susceptibility of MBL-producing Enterobacterales to meropenem in combination with investigational MBLIs

Article Snippet: Supplementation of meropenem with the most potent MBLI, 272, resulted in a 32-fold reduction in meropenem MIC for the MDR strain K. pneumoniae NCTC 13443, indicating that the compound retains the ability to enhance susceptibility to meropenem in highly resistant organisms. table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Compound(s) MIC (mg/L) E. coli ATCC BAA-2452 (NDM-1) E. coli NCTC 13476 (IMP) K. pneumoniae ATCC BAA-2146 (NDM-1) K. pneumoniae NCTC 13440 (VIM-1) K. pneumoniae NCTC 13443 (NDM-1) K. pneumoniae NCTC 13439 (VIM-1) E. coli ATCC BAA-2469 (NDM-1) 272 >128 >128 >128 >128 >128 >128 >128 276 >128 >128 >128 >128 >128 >128 >128 364 >128 >128 >128 >128 >128 >128 >128 439 >128 >128 >128 >128 >128 >128 >128 MEM 32 128 0.5 MEM/272 0.03 (32) 0.03 (128) 0.25 (128) 0.06 (16) 4 (32) 0.06 (8) 0.06 (64) MEM/276 0.06 (16) 0.03 (128) 0.5 (64) 0.06 (16) 16 (8) 0.06 (8) 0.06 (64) MEM/364 0.06 (16) 0.03 (128) 2 (16) 0.06 (16) 8 (16) 0.12 (4) 0.12 (32) MEM/439 0.06 (16) 0.06 (64) 2 (16) 0.25 (4) 16 (8) 0.25 (2) 0.06 (64) IPM 8 16 8 32 16 IPM/272 1 (8) 0.5 (8) 1 (16) 1 (8) nd nd nd IPM/276 0.5 (16) 0.25 (16) 1 (16) 1 (8) nd nd nd Open in a separate window MICs of meropenem determined in the presence or absence of MBLI compounds (4 mg/L); type of MBL noted beneath strain name.

Techniques: